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High Quality Proteinase K Powder

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  • Product Description
  • Video
  • Specification
    Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, this enzyme is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
    Features
    Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
    High activity and exceptional purity (Molecular Biology Grade & NGS Grade).
    Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal
    for digesting proteins in variety of applications.
    Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
    Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
    Available as powder, lyophilized “cake” or liquid.

    Applications:
    Extraction of DNA and RNA from different starting materials.
    Purification of target material from contaminating proteins.
    Removal of DNases and RNases during nucleic acids isolation.

    Units
    One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.
    Proteinase K – Lyophilized powder, NGS Grade
    DNA content : ≤ 0.1 pg/mg by qPCR

    Solubility in water : ≥ 50 mg/ml

    Activity
    ≥ 35 U/mg lyophilizate
    ≥ 45 U/mg protein

    Protein content : ≥ 70% Protein content is determined by measuring absorbance at 280 nm.

    Stock solution preparation 20-50 mg/ml
    solutions: use purified water for immediate use, or 50% glycerol in purified water (v/v) for long-term storage at -20˚C. < 20 mg/ml solutions: use 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 for immediate use, or 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 , 50% glycerol for long-term storage at -20˚C.
    Proteinase K is a powerful proteolytic enzyme isolated from Candida albicans. It has a high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA.

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